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Title: Evaluation of a dual-probe real time PCR system for detection of mandarin in commercial orange juice
Authors: Angel Pardo, Miguel
Citation: FOOD CHEMISTRY, 2015, 172, 377-384
Abstract: A dual-probe real time PCR assay, based on the simultaneous detection of two TaqMan (R) probes, was evaluated for the detection of mandarin in orange juice. A single conserved polymorphism, located at the 314 position of intron belongs to chloroplast trnL gene, was confirmed by sequencing in 30 mandarin, 28 orange cultivars and 13 hybrids. The assay was also successfully evaluated in a blind trial against analysing 60 samples from different industrial processes in different countries around the world. The detection limit of the assay was established in 1\% presence of mandarin detectable in processed orange juice and with a 100\% precision. The quantitative application of the assay on citrus mixtures was also investigated, pointing out that the number of chloroplast DNA copies is too variable for its possible use as quantitative analysis. This assay can be employed as a routine methodology to control the accidental mixing during industrial processes and to deter intentional fraud. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords: Real time PCR; Orange juice; Mandarin; Citrus; Adulteration; Traceability; Food quality; CHIP CAPILLARY-ELECTROPHORESIS; CHLOROPLAST NUMBER; DNA; AUTHENTICATION; IDENTIFICATION; ASSAY; QUANTIFICATION; PRODUCTS; GENETICS; PRIMERS
Issue Date: 2015
Publisher: ELSEVIER SCI LTD
Type: Article
Language: English
DOI: 10.1016/j.foodchem.2014.09.096
URI: http://dspace.azti.es/handle/24689/260
ISSN: 0308-8146
E-ISSN: 1873-7072
Funder: Spanish National Institute for Agricultural and Food Research \[RTA2007-00059-00-00]
Appears in Publication types:Artículos científicos



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