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Title: Zebrafish Axenic Larvae Colonization with Human Intestinal Microbiota
Authors: Arias-Jayo, Nerea; Alonso-Saez, Laura; Ramirez-Garcia, Andoni; Pardo, Miguel A.
Abstract: The human intestine hosts a vast and complex microbial community that is vital for maintaining several functions related with host health. The processes that determine the gut microbiome composition are poorly understood, being the interaction between species, the external environment, and the relationship with the host the most feasible. Animal models offer the opportunity to understand the interactions between the host and the microbiota. There are different gnotobiotic mice or rat models colonized with the human microbiota, however, to our knowledge, there are no reports on the colonization of germ-free zebrafish with a complex human intestinal microbiota. In the present study, we have successfully colonized 5 days postfertilization germ-free zebrafish larvae with the human intestinal microbiota previously extracted from a donor and analyzed by high-throughput sequencing the composition of the transferred microbial communities that established inside the zebrafish gut. Thus, we describe for first time which human bacteria phylotypes are able to colonize the zebrafish digestive tract. Species with relevant interest because of their linkage to dysbiosis in different human diseases, such as Akkermansia muciniphila, Eubacterium rectale, Faecalibacterium prausnitzii, Prevotella spp., or Roseburia spp. have been successfully transferred inside the zebrafish digestive tract.
Keywords: zebrafish; human microbiota; gnotobiotic; metabacoding; LACTIC-ACID BACTERIA; GUT MICROBIOTA; ULCERATIVE-COLITIS; FECAL MICROBIOTA; RIBOSOMAL-RNA; DISEASE; PHYSIOLOGY; SYMBIOSIS; OBESITY; HEALTH
Publisher: MARY ANN LIEBERT, INC
Type: Article; Early Access
Language: English
DOI: 10.1089/zeb.2017.1460
URI: http://dspace.azti.es/handle/24689/394
ISSN: 1545-8547
E-ISSN: 1557-8542
Funder: LIFE+ program (project ENVIPHAGE) [LIFE13 ENV/ES/001048]
Department of Environment, Territorial Planning, Agriculture and Fisheries of the Basque Country Government
Ramon y Cajal Fellowship by the Spanish Ministry of Economy and Competitiveness
European Commission
Appears in Publication types:Artículos científicos



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