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Title: Development of a multiplex PCR-ELISA method for the genetic authentication of Thunnus species and Katsuwonus pelamis in food products
Authors: Santaclara, Francisco J.; Velasco, Amaya; Perez-Martin, Ricardo I.; Rey-Mendez, Manuel; Angel Pardo, Miguel; Sotelo, Carmen G.; Quinteiro, Javier; Jimenez, Elisa
Citation: FOOD CHEMISTRY, 2015, 180, 9-16
Abstract: In the present work a PCR-ELISA technique for the authentication of Thunnus species was developed. This method is composed by four systems that can be used in a hierarchical way allowing the identification of several scombroids species; or each individual system independently. The hierarchical strategy, proposes a first step, to assign one sample to the Thunnus genus. Next, if the result is positive, several tests can be applied to assign the sample to some particular species of the Thunnus genus. In the case that the result is negative (absence of Thunnus species), it is possible to verify if Katsuwonus pelamis is included in the sample. The method even allows the detection of mixtures of these species in relatively low amounts (up to 1\%). Finally, this method was applied to 11 commercial samples to verify the labelling status of tuna products in the market, detecting that 18\% were mislabelling. (C) 2015 Published by Elsevier Ltd.
Keywords: PCR-ELISA; Thunnus genus; Genetic authentication; Cytochrome b gene; Cyt b; Cytochrome oxidase subunit I; COXI; POLYMERASE-CHAIN-REACTION; CYTOCHROME-B GENE; MITOCHONDRIAL-DNA SEQUENCES; SEAFOOD PRODUCTS; RFLP ANALYSIS; CANNED TUNA; VIRAL GENOMES; IDENTIFICATION; FINS; OMMASTREPHIDAE
Issue Date: 2015
Publisher: ELSEVIER SCI LTD
Type: Article
Language: English
DOI: 10.1016/j.foodchem.2014.11.076
URI: http://dspace.azti.es/handle/24689/226
ISSN: 0308-8146
E-ISSN: 1873-7072
Funder: Ministerio de Ciencia e Innovacion (Spain) \[AGL2009-13711-C03-01]
Appears in Publication types:Artículos científicos



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