Please use this identifier to cite or link to this item: http://dspace.azti.es/handle/24689/1145
Files in This Item:
There are no files associated with this item.
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBang-Andreasen, Toke
dc.contributor.authorAnwar, Muhammad Zohaib
dc.contributor.authorLanzen, Anders and Kjoller, Rasmus
dc.contributor.authorRonn, Regin
dc.contributor.authorEkelund, Flemming
dc.contributor.authorJacobsen, Carsten Suhr
dc.date.accessioned2021-07-02T08:13:19Z-
dc.date.available2021-07-02T08:13:19Z-
dc.date.issued2020
dc.identifierISI:000526682400005
dc.identifier.issn0168-6496
dc.identifier.urihttp://dspace.azti.es/handle/24689/1145-
dc.description.abstractRecycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied total RNA sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, total RNA sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.
dc.language.isoEnglish
dc.publisherOXFORD UNIV PRESS
dc.subjectmetatranscriptomics
dc.subjecttotal RNA
dc.subjectwood ash
dc.subjectbiodiversity
dc.subjectsoil biota
dc.subjectprotozoa
dc.subjectBACTERIAL COMMUNITIES
dc.subjectGEN. NOV.
dc.subjectNUTRIENT-UPTAKE
dc.subjectPH
dc.subjectFUNGAL
dc.subjectGROWTH
dc.subjectFERTILIZATION
dc.subjectDIVERSITY
dc.subjectPROTOZOA
dc.subjectRESPIRATION
dc.titleTotal RNA sequencing reveals multilevel microbial community changes and functional responses to wood ash application in agricultural and forest soil
dc.typeArticle
dc.identifier.journalFEMS MICROBIOLOGY ECOLOGY
dc.format.volume96
dc.contributor.funder`Center for Bioenergy Recycling (ASHBACK)' project
dc.contributor.funderDanish Council for Strategic ResearchDanske Strategiske Forskningsrad (DSF) [0603-00587B]
dc.contributor.funderDanish Geocenter [5298507]
dc.contributor.funderJuan de la Cierva scholarship from the Spanish Government [FJCI-2014-19572]
dc.contributor.funderEuropean UnionEuropean Commission [675546]
dc.contributor.funderDanish Council for Independent ResearchDet Frie Forskningsrad (DFF) [DFF-4002-00274]
dc.identifier.e-issn1574-6941
dc.identifier.doi10.1093/femsec/fiaa016
Appears in Publication types:Artículos científicos



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.