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dc.contributor.authorGarmendia, Maialen
dc.contributor.authorRevilla, Marta
dc.contributor.authorZarauz, Lucia
dc.date.accessioned2019-05-17T08:47:48Z-
dc.date.available2019-05-17T08:47:48Z-
dc.date.issued2013
dc.identifierISI:000314693600016
dc.identifier.citationHYDROBIOLOGIA, 2013, 704, 231-252
dc.identifier.issn0018-8158
dc.identifier.urihttp://dspace.azti.es/handle/24689/546-
dc.description.abstractA simple automatic method has been tested for biological quality assessment in coastal and estuarine waters. Samples collected in routine campaigns along the Basque coast (northern Spain) were analysed by both, a traditional microscopy technique (Utermohl) and the flow cytometer and microscope (FlowCAM). The FlowCAM was operated in AutoImage mode (i.e. without fluorescence measurements). Two different FlowCAM procedures were carried out in two campaigns separately (2005 and 2008). These differed mainly in the lower size-threshold of the particles sampled: 10 mu m in 2005, and 4 mu m in 2008. The degree of divergence was evaluated, recognizing that the Utermohl method and the FlowCAM (as employed here) count differently (i.e. phytoplankton cells and particles, respectively). In general, higher concentrations were observed with the FlowCAM (although there were some exceptions in 2005). Relationships between physico-chemical and FlowCAM variables were studied and patterns of natural variability were identified. However, a clear response to nutrient pressure was not found. It is concluded that the potential of this automatic method for deriving cost-effective indicators is limited, especially in estuaries, due to the high interference with suspended material. Further studies incorporating fluorescence measurements with the FlowCAM may be more promising. The standardization of procedures is highly recommended.
dc.language.isoeng
dc.publisherSPRINGER
dc.subjectEutrophication
dc.subjectEuropean Directives
dc.subjectPhytoplankton
dc.subjectAutomatic techniques
dc.subjectFlowCAM
dc.subjectCOASTAL MARINE ECOSYSTEMS
dc.subjectCOUNTRY NORTHERN SPAIN
dc.subjectMETHODOLOGICAL APPROACH
dc.subjectECOLOGICAL STATUS
dc.subjectPHYTOPLANKTON COMMUNITIES
dc.subjectPLANKTON DISTRIBUTION
dc.subjectENVIRONMENTAL STATUS
dc.subjectTIDAL TRIBUTARIES
dc.subjectCHESAPEAKE BAY
dc.subjectEUTROPHICATION
dc.titleTesting the usefulness of a simple automatic method for particles abundance and size determination to derive cost-effective biological indicators in large monitoring networks
dc.typeArticle
dc.identifier.journalHYDROBIOLOGIA
dc.format.page231-252
dc.format.volume704
dc.contributor.funderWater Agency (Uragentzia) of the Basque Government
dc.contributor.funderPlan National [CTM2006-09583]
dc.contributor.funderEuropean Regional Development Fund (FEDER)
dc.contributor.funderSpanish Ministry of Education and Science (MEC)
dc.contributor.funderWISER project (Water bodies in Europe: Integrative Systems to assess Ecological status and Recovery)
dc.contributor.funderEuropean Union [226273]
dc.contributor.funderDepartment of Education, Universities and Investigation of the Basque Government
dc.identifier.doi10.1007/s10750-012-1400-x
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