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Title: Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI)
Authors: Aylagas, Eva; Borja, Angel; Rodriguez-Ezpeleta, Naiara
Abstract: Marine ecosystem protection and conservation initiatives rely on the assessment of ecological integrity and health status of marine environments. The AZTI's Marine Biotic Index (AMBI), which consists on using macroinvertebrate diversity as indicator of ecosystem health, is used worldwide for this purpose. Yet, this index requires taxonomic assignment of specimens, which typically involves a time and resource consuming visual identification of each sample. DNA barcoding or metabarcoding are potential harmonized, faster and cheaper alternatives for species identification, although the suitability of these methods for easing the implementation of the AMBI is yet to be evaluated. Here, we analyze the requirements for the implementation of a genetics based AMBI (gAMBI), and show, using available sequence data, that information about presence/absence of the most frequently occurring species provides accurate AMBI values. Our results set the basics for the implementation of the gAMBI, which has direct implications for a faster and cheaper marine monitoring and health status assessment.
Issue Date: 2014
Type: Article
Language: English
DOI: 10.1371/journal.pone.0090529
ISSN: 1932-6203
Funder: Fundacion Centros Tecnologicos through an Inaki Goenaga doctoral grant
DEVOTES (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing good Environmental Status)
European Union [308392]
Basque Water Agency (URA)
Appears in Publication types:Artículos científicos

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