Please use this identifier to cite or link to this item:
Files in This Item:
File SizeFormat 
metabarcoding marine.pdf1,18 MBAdobe PDFView/Open
Title: Metabarcoding of marine zooplankton: prospects, progress and pitfalls
Authors: Bucklin, Ann; Lindeque, Penelope K.; Rodriguez-Ezpeleta, Naiara; Lehtiniemi, Maiju; Albaina, Aitor
Citation: JOURNAL OF PLANKTON RESEARCH, 2016, 38, 393-400
Abstract: Metabarcoding (large-scale taxonomic identification of complex samples via analysis of one or few orthologous DNA regions, called barcodes) is revolutionizing analysis of biodiversity of marine zooplankton assemblages. Metabarcoding relies on high-throughput DNA sequencing (HTS) technologies, which yield millions of DNA sequences in parallel and allow large-scale analysis of environmental samples. Metabarcoding studies of marine zooplankton have used various regions of nuclear small- (18S) and large-subunit (28S) rRNA, which allow accurate classification of novel sequences and reliable amplification with consensus primers, but-due to their relatively conserved nature-may underestimate species diversity in a community. To discriminate species, more variable genes are needed. A limited number of metabarcoding studies have used mitochondrial cytochrome oxidase I (COI), which ensures detection of species-level diversity, but may require group-specific primers and thus result in inconsistent amplification success rates. Reference databases with sequences for accurately-identified species are critically needed to allow taxonomic designation of molecular operational taxonomic units (MOTU) and comparison with previous studies of zooplankton diversity. Potential and promising applications of metabarcoding include rapid detection of impacts of climate change, monitoring and assessment of ecosystem health, calculation of biotic indices, characterization of food webs and detection of introduced, non-indigenous species.
Issue Date: 2016
Type: Article
Language: English
DOI: 10.1093/plankt/fbw023
ISSN: 0142-7873
E-ISSN: 1464-3774
Funder: International Council for the Exploration of the Sea (ICES)
NOAA Office of Ocean Exploration and Research \[NA15OAR0110209]
Department for Environment, Food and Rural Affairs \[NE/L003279/1]
DEVOTES (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing Good Environmental Status) project - European Union (EU) 7th Framework Program \[308392]
Basque Government \[IT558-10]
BONUS BIO-C3 project
BONUS \[Art 185]
Finnish Academy \[273660]
UK Natural Environment Research Council
Appears in Publication types:Artículos científicos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.