Please use this identifier to cite or link to this item: http://dspace.azti.es/handle/24689/981
Files in This Item:
There are no files associated with this item.
Title: Differential decay and prediction of persistence of Enterococcus spp. and Escherichia coli culturable cells and molecular markers in freshwater and seawater environments
Authors: Sagarduy, Maialen; Courtois, Sophie; Del Campo, Andrea; Mikel Garmendia, Joxe; Petrau, Agnes
Citation: INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 2019, 222, 695-704
Abstract: To quantify the impact of fecal pollution on the microbiological bathing water quality, predictive modeling is being increasingly used in which the decay rate of the fecal indicators plays an important role. The decay of sewage-sourced enterococci and Escherichia coli culturable cells and their associated molecular markers (16SrRNA) quantified by Quantitative Reverse transcription PCR were measured in controlled microcosms as well in in situ conditions using different water types, from marine waters to fresh waters with intermediate salinity. All bacterial decays were fitted to a first order decay model. In the laboratory study, the light radiation was the most influent factor affecting E. coli and enterococci survival by culture methods although environmental conditions weakly impacted the decay of molecular markers. The results also indicated differential persistence of genetic markers and culturable organisms of fecal indicator bacteria in different water systems. For each bacteria indicator and analytical method, four equations were obtained to predict the time required to have a 90\% reduction (T90) according irradiance, salinity and temperature parameters. The weighted model RMSE (Root Mean Square Error) calculated for all field experiments showed that quantification obtained with the equations defined by laboratory-based study compared reasonably well with in-situ observed quantification (0.4 and 0.2 log by standard culture methods for E. coli and Enterococcus spp. and 0.6 and 0.3 log by RT-qPCR for E. coli and Enterococcus spp. respectively). The modeling tool can be used to predict the presence of fecal pollution in marine and fresh waters in combination with either culture based- or rapid molecular methods.
Keywords: Bathing water; Prediction; Persistence; Decay; E. coli; Enterococci; 16SrRNA; FECAL INDICATOR BACTERIA; BATHING WATER; ENTERIC BACTERIA; COASTAL WATER; IN-SITU; INACTIVATION; SURVIVAL; QUALITY; ESTUARINE; QPCR
Issue Date: 2019
Publisher: ELSEVIER GMBH
Type: Article
Language: 
DOI: 10.1016/j.ijheh.2019.04.011
URI: http://dspace.azti.es/handle/24689/981
ISSN: 1438-4639
E-ISSN: 1618-131X
Funder: European Union ERDF funds (European Regional Development Fund)European Union (EU) [2016-2862410]
Region Nouvelle Aquitaine (France)
Appears in Publication types:Artículos científicos



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.