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Title: Treated and untreated wastewater effluents alter river sediment bacterial communities involved in nitrogen and sulphur cycling
Authors: Martinez-Santos, Miren; Lanzen, Anders; Unda-Calvo, Jessica and Martin, Iker; Garbisu, Carlos; Ruiz-Romera, Estilita
Citation: SCIENCE OF THE TOTAL ENVIRONMENT, 2018, 633, 1051-1061
Abstract: Studying the dynamics of nitrogen and sulphur cycling bacteria in river surface sediments is essential to better understand their contribution to global biogeochemical cycles. Evaporitic rocks settled at the headwater of the Deba River catchment (northern Spain) lead to high values of sulphate concentration in its waters. Besides, the discharge of effluents from untreated and treated residual (urban and industrial) wastewaters increases the concentration of metals, nutrients and organic compounds in its mid- and low-water courses. The aim of this study was to assess the impact of anthropogenic contamination from untreated and treated residual and industrial wastewaters on the structure and function of bacterial communities present in surface sediments of the Deba River catchment. The application of a quantitative functional approach (qPCR) based on denitrification genes (nir: nirS + nirK; and nosZ), together with a 16S rRNA gene metabarcoding structural analysis, revealed (i) the high relevance of the sulphur cycle at headwater surface sediments (as reflected by the abundance of members of the Syntrophobacterales order, and the Sulfuricurvum and Thiobacillus genera) and (ii) the predominance of sulphide-driven autotrophic denitrification over heterotrophic denitrification. Incomplete heterotrophic denitrification appeared to be predominant in surface sediments strongly impacted by treated and untreated effluents, as reflected by the lower values of the nosZ/nir ratio, thus favouring N2O emissions. Understanding nitrogen and sulphur cycling pathways has profound implications for the management of river ecosystems, since this knowledge can help us determine whether a specific river is acting or not as a source of greenhouse gases (i.e., N2O). (C) 2018 Elsevier B.V. All rights reserved.
Keywords: Autotrophic/heterotrophic denitrification; Nitrogen and sulphur cycling; Metabarcoding; qPCR (nir and nosZ); River surface sediments; SURFACE BOTTOM SEDIMENTS; MICROBIAL COMMUNITIES; OXIDE REDUCTASE; NITRATE REDUCTION; URBAN CATCHMENT; TREATMENT-PLANT; ORGANIC-MATTER; N2O EMISSIONS; GEN. NOV.; DIVERSITY
Issue Date: 2018
Type: Article
Language: English
DOI: 10.1016/j.scitotenv.2018.03.229
ISSN: 0048-9697
E-ISSN: 1879-1026
Funder: Ministry of Economy and Competitiveness [CTM2014-55270-R]
Basque Government (Consolidated Group of Hydrogeology and Environment) [IT1029-16]
University of the Basque Country (UPV-EHU) [UFI11/26]
Appears in Publication types:Artículos científicos

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