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Título : High-Fat Diet Consumption Induces Microbiota Dysbiosis and Intestinal Inflammation in Zebrafish
Autor : Arias-Jayo, Nerea; Abecia, Leticia; Alonso-Saez, Laura; Ramirez-Garcia, Andoni; Rodriguez, Alfonso; Pardo, Miguel Angel
Citación : MICROBIAL ECOLOGY, 2018, 76, 1089-1101
Resumen : Energy-dense foods and overnutrition represent major starting points altering lipid metabolism, systemic inflammation and gut microbiota. The aim of this work was to investigate the effects of a high-fat diet (HFD) over a period of 25days on intestinal microbiota and inflammation in zebrafish. Microbial composition of HFD-fed animals was analysed and compared to controls by 16S rRNA sequencing and quantitative PCR. The expression level on several genes related to inflammation was tested. Furthermore, microscopic assessment of the intestine was performed in both conditions. The consumption of the HFD resulted in microbial dysbiosis, characterised by an increase in the relative abundance of the phylum Bacteroidetes. Moreover, an emerging intestinal inflammation via NF- activation was confirmed by the overexpression of several genes related to signalling receptors, antimicrobial metabolism and the inflammatory cascade. The intestinal barrier was also damaged, with an increase of goblet cell mucin production. This is the first study performed in zebrafish which suggests that the consumption of a diet enriched with 10\% fat changes the intestinal microbial community composition, which was correlated with low-grade inflammation.
Palabras clave : Zebrafish; Microbiota; Inflammation; High-fat diet; TOLL-LIKE RECEPTORS; GUT MICROBIOTA; GNOTOBIOTIC ZEBRAFISH; DANIO-RERIO; IMMUNE-SYSTEM; OBESITY; MICE; ACTIVATION; EXPRESSION; COLITIS
Fecha de publicación : 2018
Editorial : SPRINGER
Tipo de documento: Article
Idioma: Inglés
DOI: 10.1007/s00248-018-1198-9
URI : http://dspace.azti.es/handle/24689/772
ISSN : 0095-3628
E-ISSN: 1432-184X
Patrocinador: MAROMEGA Project (Basque Country Government, Economic Development and Structure)
Sira Carrasco scholarship (Spanish Society for Paediatric Gastroenterology, Hepatology and Nutrition)
Ramon y Cajal program of the Spanish Ministry of Economy and Competitiveness (MINECO)
Basque Country Agriculture, Fisheries and Food Department
Aparece en las tipos de publicación: Artículos científicos



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