Please use this identifier to cite or link to this item:
Files in This Item:
There are no files associated with this item.
Title: Altered Red Blood Cell Membrane Fatty Acid Profile in Cancer Patients
Authors: Amezaga, Javier; Arranz, Sara; Urruticoechea, Ander and Ugartemendia, Gurutze; Larraioz, Aitziber; Louka, Maria and Uriarte, Matxalen; Ferreri, Carla; Tueros, Itziar
Abstract: The fatty acid (FA) composition of red blood cell (RBC) membrane phospholipids of cancer patients can reflect tumor status, dietary intakes, and cancer type or therapy. However, the characteristic membrane profiles have so far not yet defined as a potential biomarker to monitor disease evolution. The present work provides the first evidence of cancer metabolic signatures affecting cell membranes that are independent of nutritional habits. From the Oncology Outpatient Unit of the Onkologikoa hospital, two groups of cancer patients (n = 54) and healthy controls (n = 37) were recruited, and mature RBCs membrane phospholipids were analyzed for FA profiling (GC-MS). Dietary habits were evaluated using a validated food frequency questionnaire. The adjusted Analysis of Covariance Test (ANCOVA) model revealed cancer patients to have a lower relative percentage of saturated fatty acids (SFA) (C16:0 (5.7\%); C18:0 (15.9\%)), and higher monounsaturated fatty acids (MUFA) (9c-C18:1 (12.9\%) and 11c-C18:1 (54.5\%)), compared to controls. In line with this, we observe that the desaturase enzymatic index (delta-9 desaturase (9D), +28.3\%) and the membrane saturation index (SI = SFA/MUFA; -27.3\%) were similarly modulated. Polyunsaturated fatty acids (PUFA) families showed an increase of n-6 C18:2 and C20:3 (15.7\% and 22.2\% respectively), with no differences in n-6 C20:4 and n-3 PUFA (docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)). Importantly, these changes were found independent of foods and fat intakes from the diet. The membrane lipid profile in RBC was useful to ascertain the presence of two main metabolic signatures of increased desaturation activity and omega-6 in cancer patients, statistically independent from dietary habits.
Keywords: cancer; membrane lipidome; red blood cell; unsaturated fatty acids; saturation index; desaturase index; STEAROYL-COA DESATURASE-1; FUNCTIONAL LIPIDOMICS; METABOLISM; RISK; DIET; NUTRITION; OBESITY
Issue Date: 2018
Publisher: MDPI
Type: Article
Language: English
DOI: 10.3390/nu10121853
ISSN: 2072-6643
Funder: Basque Country Government (Department of the Environment, Regional Planning, Agriculture and Fisheries)
Lipinutragen srl [MSCA-ITN-2014-ETN]
Appears in Publication types:Artículos científicos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.