Mesedez, erabili identifikatzaile hau item hau aipatzeko edo estekatzeko: http://dspace.azti.es/handle/24689/678
Item honetako fitxategiak:
Fitxategia TamainaFormatua 
Identification Saccharomyces.pdf656,19 kBAdobe PDFBistaratu/Ireki
Titulua: Identification of a Saccharomyces cerevisiae Glucosidase That Hydrolyzes Flavonoid Glucosides
Egilea: Schmidt, Sabine; Rainieri, Sandra; Witte, Simone; Matern, Ulrich; Martens, Stefan
Zitazioa: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77, 1751-1757
Laburpena: Baker's yeast (Saccharomyces cerevisiae) whole-cell bioconversions of naringenin 7-O-beta-glucoside revealed considerable beta-glucosidase activity, which impairs any strategy to generate or modify flavonoid glucosides in yeast transformants. Up to 10 putative glycoside hydrolases annotated in the S. cerevisiae genome database were overexpressed with His tags in yeast cells. Examination of these recombinant, partially purified polypeptides for hydrolytic activity with synthetic chromogenic alpha- or beta-glucosides identified three efficient beta-glucosidases (EXG1, SPR1, and YIR007W), which were further assayed with natural flavonoid beta-glucoside substrates and product verification by thin-layer chromatography (TLC) or high-performance liquid chromatography ( HPLC). Preferential hydrolysis of 7- or 4'-O-glucosides of isoflavones, flavonols, flavones, and flavanones was observed in vitro with all three glucosidases, while anthocyanins were also accepted as substrates. The glucosidase activities of EXG1 and SPR1 were completely abolished by Val168Tyr mutation, which confirmed the relevance of this residue, as reported for other glucosidases. Most importantly, biotransformation experiments with knockout yeast strains revealed that only EXG1 knockout strains lost the capability to hydrolyze flavonoid glucosides.
Gako-hitzak: CYTOSOLIC BETA-GLUCOSIDASE; CELL-SUSPENSION CULTURES; SYNTHASE-I; PETROSELINUM-HORTENSE; ASPERGILLUS-ORYZAE; ESCHERICHIA-COLI; NATURAL-PRODUCTS; YEAST; PURIFICATION; CLONING
Gordailuaren-data: 2011
Argitalpen: AMER SOC MICROBIOLOGY
Dokumentu mota: Article
Hizkuntza: Ingelesa
DOI: 10.1128/AEM.01125-10
URI: http://dspace.azti.es/handle/24689/678
ISSN: 0099-2240
E-ISSN: 1098-5336
Babeslea: Deutsche Forschungsgemeinschaft (DFG)
Bildumetan azaltzen da:Artículos científicos



DSpaceko itemak copyright bidez babestuta daude, eskubide guztiak gordeta, baldin eta kontrakoa adierazten ez bada.