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Title: cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15 CLNA Mixture Activates PPAR alpha in HEK293 and Reduces Triacylglycerols in 3T3-L1 cells
Authors: Miranda, Jonatan; Lasa, Arrate; Fernandez-Quintela, Alfredo; Ayo, Josune; Dentin, Renaud; Portillo, Maria P.; Garcia-Marzo, Cristina
Citation: LIPIDS, 2011, 46, 1005-1012
Abstract: Scientific research is constantly working to find new molecules that are effective in preventing excessive accumulation of body fat. The aim of the present work was to assess the potential agonism on PPAR alpha and PPAR gamma of a conjugated linolenic acid (CLNA) isomer mixture, consisting of two CLNA isomers (cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15). Secondly, we aimed to analyze the effects of this mixture on triacylglycerol accumulation in 3T3-L1 mature adipocytes. Luciferase transactivation assay was used to analyze whether the CLNA mixture activated PPARs. The expression of several enzymes and transcriptional factors involved in the main metabolic pathways that control triacylglycerol accumulation in adipocytes was assessed by real time RT-PCR in 3T3-L1 adipocytes treated for 20 h with the CLNA mixture. The mixture activated PPRE in cells with PPAR alpha receptor over-expression, but not those with PPAR gamma over-expression. Decreased triacylglycerol was found in treated adipocytes. The lowest dose (10 mu M) increased HSL expression and the highest dose (100 mu M) increased ATGL gene expression. The other genes analyzed remained unchanged. The hypothesis of an anti-obesity action of the analyzed CLNA mixture, based on increased lipid mobilization in adipose tissue, can be proposed.
Keywords: PPAR alpha; PPAR gamma; Conjugated linolenic acid; HSL; ATGL; 3T3-L1; CONJUGATED LINOLEIC-ACID; LICORICE FLAVONOID OIL; INDUCED OBESE RATS; PEROXISOME PROLIFERATOR; LIPID-METABOLISM; BODY-COMPOSITION; ADIPOSE-TISSUE; INSULIN SENSITIVITY; GLUCOSE-TOLERANCE; GENE-EXPRESSION
Issue Date: 2011
Publisher: SPRINGER HEIDELBERG
Type: Article
Language: English
DOI: 10.1007/s11745-011-3615-4
URI: http://dspace.azti.es/handle/24689/646
ISSN: 0024-4201
Funder: Government of Pais Vasco [IT-386-10, CTP09/R5]
Instituto de Salud Carlos III (RETIC PREDIMED)
CYTED [208RT0343]
Ministerio de Educacion y Ciencia
Appears in Publication types:Artículos científicos



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