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dc.contributor.authorHernandez, Igor
dc.date.accessioned2018-06-29T10:20:54Z-
dc.date.available2018-06-29T10:20:54Z-
dc.date.issued2017
dc.identifierISI:000398435100001
dc.identifier.issn1664-302X
dc.identifier.urihttp://dspace.azti.es/handle/24689/467-
dc.description.abstractBacteria of the genus Serratia, mainly S. proteamaculans and S. fonticola, are important spoilage agents in Atlantic horse mackerel (Trachurus trachurus). In order to evaluate whether bacteriophages against Serratia could delay the spoilage process, 11 viral strains active against this genus were isolated from food and best candidate was applied to fresh mackerel filets. All the phages belong to the Siphoviridae and Podoviridae families and were active at multiplicity of infection (MOI) levels below 1: 1 in Long \& Hammer broth. The ability of phage AZT6 to control Serratia populations in real food was tested in Atlantic horse mackerel extract and applied to fresh mackerel filets. Treatment with high phage concentration (MOI 350: 1, initial Serratia population 3.9 +/- 0.3 Log cfu/g) can reduce the Serratia populations up to 90\% during fish storage (a maximum of 6 days) at low temperatures (6 degrees C). Bacterial inhibition was dependent on the bacteriophage dosage, and MOI of 10: 1 or lower did not significantly affect the Serratia populations.
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SA
dc.subjectphagotherapy
dc.subjectSerratia
dc.subjectfish spoilage
dc.subjectshelf life
dc.subjectTrachurus trachurus
dc.subjectLISTERIA-MONOCYTOGENES
dc.subjectPHAGE THERAPY
dc.subjectMARCESCENS
dc.subjectBIOCONTROL
dc.subjectSTORAGE
dc.subjectMICROENCAPSULATION
dc.subjectBACTERIOCINS
dc.subjectSALMONELLA
dc.subjectMICROBIOTA
dc.subjectGROWTH
dc.titleBacteriophages against Serratia as Fish Spoilage Control Technology
dc.typeArticle
dc.identifier.journalFRONTIERS IN MICROBIOLOGY
dc.format.volume8
dc.contributor.funderBasque Government (Department of the Environment, Regional Planning, Agriculture and Fisheries)
dc.contributor.funderLIFE C program [LIFE13 ENV/ES/001048 ENVIPHAGE]
dc.identifier.doi10.3389/fmicb.2017.00449
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