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Title: Molecular cloning and measurement of telomerase reverse transcriptase (TERT) transcription patterns in tissues of European hake (Merluccius merluccius) and Atlantic cod (Gadus morhua) during aging
Authors: Lopez de Abechuco, E.; Bilbao, E.; Soto, M.; Diez, G.
Citation: GENE, 2014, 541, 8-18
Abstract: Telomerase is a reverse transcriptase ribonucleoprotein that maintains the ends of linear chromosomes. This enzyme plays a major role in cell processes like proliferation, differentiation and tumorigenesis, being associated with aging and survival of species. In this study, the gene coding for TERT (Telomerase Reverse Transcriptase) of two commercial fish species, European hake (Merluccius merluccius) and Atlantic cod (Gadus morhua), has been partially cloned. A fragment of 1581 bp (hake) and 633 bp (cod) showed high homology (identity 74\%, query cover 99\%, E-value = 0) with known Perciformes TERT sequences. TERT transcription patterns were assessed by qRT-PCR in different tissues of hake (brain, ovary, testis, muscle, skin, gills, liver and kidney) and cod (brain, muscle and skin) of different sizes/ages in order to understand its role in the physiological aging of teleosts. TERT was found to be ubiquitously transcribed in all tissues and size/age groups studied in both species. Significantly higher relative transcription levels (p < 0.05) were found with increasing size/age of M. merluccius in the kidney, muscle, skin and gonad, the latter exhibiting particularly high relative transcription levels. Male hakes showed higher TEAT relative transcription levels in the brain, gonad and liver than females, although these differences were not statistically significant (p < 0.05). In G. morhua, higher TERT relative transcription levels were recorded in the muscle and brain of fry and juvenile individuals. Therefore, TERT relative transcription pattern exhibited a higher telomerase demand in early developmental stages and also in mature stages, suggesting tissue renewal or regeneration processes as a conserved mechanism for maintaining long-term cell proliferation capacity and preventing senescence. Thus, it can be concluded that TERT relative transcription level was species and tissue specific and changed with the age of fishes. (C) 2014 Elsevier B.V. All rights reserved.
Keywords: Telomerase; Aging; Hake; Cod; Telomeres; REAL-TIME PCR; CATALYTIC SUBUNIT; CELLULAR SENESCENCE; VERTEBRATE MODEL; JAPANESE MEDAKA; ORYZIAS-LATIPES; IN-VIVO; RT-PCR; LENGTH; EXPRESSION
Issue Date: 2014
Publisher: ELSEVIER SCIENCE BV
Type: Article
Language: English
DOI: 10.1016/j.gene.2014.03.006
URI: http://dspace.azti.es/handle/24689/351
ISSN: 0378-1119
E-ISSN: 1879-0038
Funder: AZTI-Tecnalia
Departamento de Agricultura, Pesca y Alimentacion of Basque Government [ATM2010telom]
Spanish Ministerio de Economia y Competitividad [CTM2010-21856-C03-03]
Inaki Goenaga Foundation (OTELOFISH)
Appears in Publication types:Artículos científicos



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