Please use this identifier to cite or link to this item:
Files in This Item:
There are no files associated with this item.
Title: A novel liquid chromatography-mass spectrometric method for the simultaneous determination of trimethylamine, dimethylamine and methylamine in fishery products
Authors: Balino-Zuazo, Lander; Barranco, Alejandro
Citation: FOOD CHEMISTRY, 2016, 196, 1207-1214
Abstract: A novel multianalyte method has been developed to determine the concentration of trimethylamine (TMA), dimethylamine (DMA) and methylamine (MA) in fish. The optimal points of the key parameters (pH 8, 60 degrees C and 60 min of reaction time) were determined for the reaction with tert-butyl bromoacetate. An HILIC column was used to elute all the compounds in only 12 min and selective quantitation of the compounds was achieved. The methodology was validated in trout, hake and Atlantic horse mackerel and was used to study the shelf life of fish under refrigeration. A good correlation with the picrate method was obtained and the proposed methodology has been proved to be robust, specific and sensible with a limit of detection of 0.08 mg N-TMA kg(-1) in freshwater fish and 1 mg N-TMA kg(-1) in marine fish. In addition, TMA has been proved to be a good spoilage indicator also in freshwater fish. (C) 2015 Elsevier Ltd. All rights reserved.
Keywords: Trimethylamine; Volatile amines; Hydrophilic interaction liquid chromatography; Liquid chromatography-mass spectrometry; Fish spoilage; FLAVIN-CONTAINING MONOOXYGENASE; N-OXIDE; URINARY TRIMETHYLAMINE; SARDINA-PILCHARDUS; SPOILAGE BACTERIA; FRESHNESS; STORAGE; ASSESSMENTS; EXTRACTS; AMMONIA
Issue Date: 2016
Type: Article
Language: English
DOI: 10.1016/j.foodchem.2015.09.086
ISSN: 0308-8146
E-ISSN: 1873-7072
Funder: Economic Development and Competitiveness Department of the Basque Government - Spain
Basque Government - Spain
Appears in Publication types:Artículos científicos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.