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dc.contributor.authorBucklin, Ann-
dc.contributor.authorLindeque, Penelope K.-
dc.contributor.authorRodriguez-Ezpeleta, Naiara-
dc.contributor.authorLehtiniemi, Maiju-
dc.contributor.authorAlbaina, Aitor-
dc.date.accessioned2017-08-23T08:52:04Z-
dc.date.available2017-08-23T08:52:04Z-
dc.date.issued2016-
dc.identifierISI:000377299000001-
dc.identifier.citationJOURNAL OF PLANKTON RESEARCH, 2016, 38, 393-400-
dc.identifier.issn0142-7873-
dc.identifier.urihttp://dspace.azti.es/handle/24689/149-
dc.description.abstractMetabarcoding (large-scale taxonomic identification of complex samples via analysis of one or few orthologous DNA regions, called barcodes) is revolutionizing analysis of biodiversity of marine zooplankton assemblages. Metabarcoding relies on high-throughput DNA sequencing (HTS) technologies, which yield millions of DNA sequences in parallel and allow large-scale analysis of environmental samples. Metabarcoding studies of marine zooplankton have used various regions of nuclear small- (18S) and large-subunit (28S) rRNA, which allow accurate classification of novel sequences and reliable amplification with consensus primers, but-due to their relatively conserved nature-may underestimate species diversity in a community. To discriminate species, more variable genes are needed. A limited number of metabarcoding studies have used mitochondrial cytochrome oxidase I (COI), which ensures detection of species-level diversity, but may require group-specific primers and thus result in inconsistent amplification success rates. Reference databases with sequences for accurately-identified species are critically needed to allow taxonomic designation of molecular operational taxonomic units (MOTU) and comparison with previous studies of zooplankton diversity. Potential and promising applications of metabarcoding include rapid detection of impacts of climate change, monitoring and assessment of ecosystem health, calculation of biotic indices, characterization of food webs and detection of introduced, non-indigenous species.-
dc.description.sponsorshipWe acknowledge support from the International Council for the Exploration of the Sea (ICES) for WGIMT, as well as conferences and symposia related to the Working Group's mission. ICES Delegates from member countries provided financial support to some WGIMT members for travel to annual meetings. Additional support was provided by: NOAA Office of Ocean Exploration and Research (Grant No. NA15OAR0110209 to AB, University of Connecticut, USA); UK Natural Environment Research Council and Department for Environment, Food and Rural Affairs (Grant No. NE/L003279/1 to PKL, Plymouth Marine Laboratory, UK); DEVOTES (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing Good Environmental Status) project funded by the European Union (EU) 7th Framework Program (Grant No. 308392 to NR-E, AZTI, Spain); Basque Government (Grant No. IT558-10 to AA, University of the Basque Country, UPV/EHU, Spain); and BONUS BIO-C3 project and BONUS (Art 185), funded by the EU and Finnish Academy (Grant No. 273660 to ML, Finnish Environment Institute, Finland).-
dc.language.isoeng-
dc.publisherOXFORD UNIV PRESS-
dc.subjectmetabarcoding-
dc.subjectDNA barcoding-
dc.subjectbiodiversity-
dc.subjecthigh-throughput DNA sequencing-
dc.subjectzooplankton-
dc.subjectMETAGENETIC COMMUNITY ANALYSIS-
dc.subjectRIBOSOMAL-RNA-
dc.subjectENVIRONMENTAL DNA-
dc.subjectCOASTAL WATERS-
dc.subjectPLANKTONIC COPEPODS-
dc.subjectBIODIVERSITY-
dc.subjectDIVERSITY-
dc.subjectABUNDANCE-
dc.subjectIDENTIFICATION-
dc.subjectSURVEILLANCE-
dc.titleMetabarcoding of marine zooplankton: prospects, progress and pitfalls-
dc.typeArticle-
dc.identifier.journalJOURNAL OF PLANKTON RESEARCH-
dc.format.page393-400-
dc.format.volume38-
dc.contributor.funderInternational Council for the Exploration of the Sea (ICES)-
dc.contributor.funderNOAA Office of Ocean Exploration and Research \[NA15OAR0110209]-
dc.contributor.funderDepartment for Environment, Food and Rural Affairs \[NE/L003279/1]-
dc.contributor.funderDEVOTES (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing Good Environmental Status) project - European Union (EU) 7th Framework Program \[308392]-
dc.contributor.funderBasque Government \[IT558-10]-
dc.contributor.funderBONUS BIO-C3 project-
dc.contributor.funderBONUS \[Art 185]-
dc.contributor.funderEU-
dc.contributor.funderFinnish Academy \[273660]-
dc.contributor.funderUK Natural Environment Research Council-
dc.identifier.e-issn1464-3774-
dc.identifier.doi10.1093/plankt/fbw023-
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