Please use this identifier to cite or link to this item:
Files in This Item:
There are no files associated with this item.
Title: RNA release via membrane vesicles in Pseudomonas aeruginosa PAO1 is associated with the growth phase
Authors: Perez-Cruz, Carla; Brianso, Ferran; Sonnleitner, Elisabeth and Blaesi, Udo; Mercade, Elena
Citation: ENVIRONMENTAL MICROBIOLOGY, 2021, 23, 5030-5041
Abstract: Pseudomonas aeruginosa PAO1 membrane vesicles (MVs) are known to play a role in cell-to-cell communication. Several studies have shown that the MV composition and physicochemical properties vary according to the bacterial growth stage, but the impact this might have on the externalization of RNA via MVs has not been addressed. Therefore, a study to characterize the RNA content from MVs retrieved at different growth phases was conducted. First, the transcriptome analyses revealed a higher abundance of around 300 RNA species in MVs when compared with the cells. The vesiculation rate along the growth curve was determined, showing that the release of MVs increased during the transition to the stationary phase, whereas it decreased in the late stationary phase. RNA-seq of MVs retrieved along the transition to the stationary phase demonstrated that the RNA cargo of vesicles did not vary. However, the amount of smaller RNAs (<200 nt) inside MVs retrieved in the late exponential phase was higher than in the stationary phase MVs. These results indicate that the externalization of RNA via MVs occurs during late exponential phase and implies the secretion of different types of MVs during growth.
Issue Date: 2021
Publisher: WILEY
Type: Article
DOI: 10.1111/1462-2920.15436
ISSN: 1462-2912
E-ISSN: 1462-2920
Funder: Government of SpainSpanish Government [CTQ2014-59632-R]
Austrian Science FundAustrian Science Fund (FWF) [P28711-B22, RNA-REG F43, AF4311]
APIF fellowship from the University of Barcelona
EMBO Short-Term fellowshipEuropean Molecular Biology Organization (EMBO) [7007]
FEMS [FEMS-RG2016-0098]
Appears in Publication types:Artículos científicos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.