Please use this identifier to cite or link to this item: http://dspace.azti.es/handle/24689/1223
Files in This Item:
There are no files associated with this item.
Title: Isolation, host specificity and genetic characterization of Campylobacter specific bacteriophages from poultry and swine sources
Authors: Nafarrate, Ibai; Mateo, Estibaliz; Miranda-Cadena, Katherine and Lasagabaster, Amaia
Abstract: The isolation and characterization of 304 Campylobacter specific bacteriophage isolates from broiler and swine sources is reported in this study. Genome size characterization determined by PFGE classified these isolates, called CAM1-CAM304, within the campylophages group II (n = 18) and group III (n = 286). Host range analyses showed a high host specificity and similar lytic spectrum among isolates of the same group. Campylophages of group II infected C. jejuni, C. coli and even a C. fetus strain whereas those of group III only infected C. jejuni strains. The most promising 59 campylophage candidates were selected according to their lytic activity and their genetic diversity was analyzed by RFLP using Smit and Hhal endonucleases for group II and III campylophages, respectively. Moreover, RAPD-PCR technique was for the first time assessed in the genetic characterization of campylophages and it was shown to be effective only for those of group II. Bacteriophage isolates grouped in a same genotype displayed different host ranges, therefore, 13 campylophages of group II and eight of group III were differentiated considering all the approaches assayed. An in-depth analysis of these bacteriophages will be performed to confirm their promising potential for the biocontrol of Campylobacter within the farm to fork process.
Keywords: Campylophage; Biocontrol; PFGE; RFLP; RAPD-PCR; Lytic spectrum; JEJUNI; COLI; PHAGES; SURFACE; RANGE
Issue Date: 2021
Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Type: Article
Language: 
DOI: 10.1016/j.fm.2021.103742
URI: http://dspace.azti.es/handle/24689/1223
ISSN: 0740-0020
E-ISSN: 1095-9998
Funder: Department of Agriculture, Fisheries and Food of the Basque Government
European Institute of Innovation and Technology (EIT), a body of the European Union, within the EIT Food program (C-SNIPER Project) [19141]
Appears in Publication types:Artículos científicos



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.