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dc.contributor.authorAlonso-Saez, Laura
dc.contributor.authorMoran, Xose Anxelu G.
dc.contributor.authorGonzalez, Jose M.
dc.date.accessioned2021-07-02T08:13:14Z-
dc.date.available2021-07-02T08:13:14Z-
dc.date.issued2020
dc.identifierISI:000526249200001
dc.identifier.issn1664-302X
dc.identifier.urihttp://dspace.azti.es/handle/24689/1140-
dc.description.abstractEnvironmental microbial gene expression patterns remain largely unexplored, particularly at interannual time scales. We analyzed the variability in the expression of marker genes involved in ecologically relevant biogeochemical processes at a temperate Atlantic site over two consecutive years. Most of nifH transcripts, involved in nitrogen (N) fixation, were affiliated with the symbiotic cyanobacterium Candidatus Atelocyanobacterium thalassa, suggesting a key role as N providers in this system. The expression of nifH and amoA (i.e., marker for ammonia oxidation) showed consistent maxima in summer and autumn, respectively, suggesting a temporal succession of these important N cycling processes. The patterns of expression of genes related to the oxidation of carbon monoxide (coxL) and reduced sulfur (soxB) were different from that of amoA, indicating alternate timings for these energy conservation strategies. We detected expression of alkaline phosphatases, induced under phosphorus limitation, in agreement with the reported co-limitation by this nutrient at the study site. In contrast, low-affinity phosphate membrane transporters (pit) typically expressed under phosphorus luxury conditions, were mainly detected in post-bloom conditions. Rhodobacteraceae dominated the expression of soxB, coxL and ureases, while Pelagibacteraceae dominated the expression of proteorhodopsins. Bacteroidetes and Gammaproteobacteria were major contributors to the uptake of inorganic nutrients (pit and amt transporters). Yet, in autumn, Thauma- and Euryarchaeota unexpectedly contributed importantly to the uptake of ammonia and phosphate, respectively. We provide new hints on the active players and potential dynamics of ecologically relevant functions in situ, highlighting the potential of metatranscriptomics to provide significant input to future omics-driven marine ecosystem assessment.
dc.language.isoEnglish
dc.publisherFRONTIERS MEDIA SA
dc.subjectgene expression
dc.subjectmetatranscriptomics
dc.subjectcoastal
dc.subjectbacterioplankton
dc.subjectfunctional marker genes
dc.subjectANOXYGENIC PHOTOTROPHIC BACTERIA
dc.subjectNW MEDITERRANEAN SEA
dc.subjectOPEN-OCEAN
dc.subjectMETABOLIC PATHWAYS
dc.subjectDIVERSITY
dc.subjectPROTEORHODOPSIN
dc.subjectDIMETHYLSULFONIOPROPIONATE
dc.subjectNITRIFICATION
dc.subjectEXPRESSION
dc.subjectGROWTH
dc.titleTranscriptional Patterns of Biogeochemically Relevant Marker Genes by Temperate Marine Bacteria
dc.typeArticle
dc.identifier.journalFRONTIERS IN MICROBIOLOGY
dc.format.volume11
dc.contributor.funderMarie Curie European Reintegration Grant FUNDIVERSITY' (FP7) [268331]
dc.contributor.funderSpanish Institute of Oceanography (IEO)
dc.contributor.funderSpanish Ministry of Economy, Industry and Competitiveness [RYC-2012-11404, CTM2016-80095C2-2-R]
dc.identifier.doi10.3389/fmicb.2020.00465
Appears in Publication types:Artículos científicos



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