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Title: Efficient isolation of Campylobacter bacteriophages from chicken skin, analysis of several isolation protocols
Authors: Nafarrate, Ibai; Mateo, Estibaliz; Amarita, Felix; Martinez de Maranon, Inigo; Lasagabaster, Amaia
Abstract: The application of Campylobacter specific bacteriophages appears as a promising food safety tool for the biocontrol of this pathogen in the poultry meat production chain. However, their isolation is a complicated challenge since their occurrence appears to be low. This work assessed the efficiency of seven protocols for recovering Campylobacter phages from chicken skin samples inoculated at phage loads from 5.0 x 10(1) to 5.0 x 10(6) PFU/g. The enrichment of chicken skin in selective Bolton broth containing target isolates was the most efficient procedure, showing a low detection limit of 5.0 x 101 PFU/g and high recovery rates of up to 560\%. This method's effectiveness increased as phage concentration decreased, showing its suitability for phage isolation. When this method was applied to isolate new Campylobacter phages from retail chicken skin, a total of 280 phages were recovered achieving an isolation success rate of 257\%. From the 109 samples 68 resulted phage positive (62\%). Chicken skin could be, therefore, considered a rich source in Campylobacter phages. This method is a simple, reproducible and efficient approach for the successful isolation of both group II and III Campylobacter specific bacteriophages, which could be helpful for the enhancement of food safety by reducing this pathogen contamination in broiler meat.
Keywords: Phage; Campylophage; Phage-therapy; Biocontrol; Isolation rate; Poultry; JEJUNI; IDENTIFICATION; PHAGES; RETAIL
Issue Date: 2020
Type: Article
DOI: 10.1016/
ISSN: 0740-0020
E-ISSN: 1095-9998
Funder: Department of Agriculture, Fisheries and Food of the Basque Government
Appears in Publication types:Artículos científicos

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